xylB [2019-06-26 09:33:40]

xylulokinase

locus: BSU17610

pI: 6.46

mw: 55.25 kDa

protein length: 499 aa

gene length: 1500 bp

function: utilization of xylan and xylose

product: xylulokinase

essential: no

ec: 2.7.1.17

synonyms: yncA

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.5|Utilization of xylan/ xylose]

Gene

Coordinates: 1,893,396 → 1,894,895

The protein

Catalyzed reaction/ biological activity

• ATP + D-xylulose = ADP + D-xylulose 5-phosphate (according to Swiss-Prot)

Protein family

• FGGY kinase family (according to Swiss-Prot)

Structure

• [PDB|2NLX] (from ''E. coli'', 35% identity, 52% similarity) [Pubmed|17123542]

Expression and Regulation

Operons

• genes: [gene|24829427B4518FE67038F194B54D4AAFAC64EF19|xylA]-[gene|F417A5B46CC7FC69DC8606A46D1AD5E41FA35B13|xylB]
  description: [Pubmed|1921970]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|2454911], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|8132469], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]
  • [protein|AF4395D134485290F4AA307B48494FED39E52CD7|XylR]: repression, [Pubmed|2454911], in [regulon|AF4395D134485290F4AA307B48494FED39E52CD7|XylR regulon]

  regulation

  • carbon catabolite repression ([protein|search|CcpA]) [Pubmed|8132469]

Biological materials

Mutant

• GP1151 (del aphA3) available in [SW|Stülke] lab
• 1A719 (no resistance), [Pubmed|1719948], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A719&Search=1A719 BGSC]
• BKE17610 (Δ[gene|F417A5B46CC7FC69DC8606A46D1AD5E41FA35B13|xylB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE17610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTTTCACTCCTTGCCC, downstream forward: _UP4_TAATGATGTTATTGTCTGGA
• BKK17610 (Δ[gene|F417A5B46CC7FC69DC8606A46D1AD5E41FA35B13|xylB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK17610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTTTCACTCCTTGCCC, downstream forward: _UP4_TAATGATGTTATTGTCTGGA

labs

• [SW|Wolfgang Hillen], Erlangen University, Germany [http://www.biologie.uni-erlangen.de/mibi/index2.html Homepage]

References

• 17123542,1921970,2454911,8132469